Chromatin Immunoprecipitation:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Flow Cytometry:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Magnetic Resonance Imaging:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Biomarker Discovery:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
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